home > faculty > snyder

MICHAEL SNYDER

Our laboratory carries out a variety of projects to analyze cell structure and division in yeast and humans. These projects fall into several areas: A) Cell biology: 1) We have been studying how a critical component involved in organization of the microtubule organizing center, gamma-tubulin, functions during the cell cycle. We have found that this protein is regulated by protein phosphorylation and that this regulation is required for proper microtubule organization. 2) We have discovered a novel cell cycle checkpoint that monitors the organization of the peripheral cytoskeleton and coordinates nuclear division with cytokskeletal organization. This checkpoint is monitored by a highly conserved signaling pathway. Current efforts are directed toward understanding the detailed mechanisms by which this checkpoint functions. B) Genomics: 1) In collaboration with Pat Brown's lab, we have developed a novel method to identify all of the chromosomal targets for transcription factors in vivo.We are currently attempting to map the targets for all transcription factors in yeast, thereby dissecting the complete circuitry of transcriptional regulation within a eucaryotic organism. 2) We have developed a novel protein chip technology and used it to analyze substrates for nearly all protein kinases of yeast. This methodology is currently being used to analyze the entire yeast proteome.

Selected Publications
Zhan, X., Lee, M. Abenes, G., VonReis, I., Kittinunvorakoon, C., Ross-Macdonald, P., Snyder, M. and Liu., F. Functional Analyses of Genes Encoded by Murine Cytomegalovirus by Transposon-Mediated Insertional Mutagenesis. Submitted

Barral, Y., Mermall, V., Mooseker, M.S. and Snyder, M. (2000). The Septins Form a Barrier Necessary for the Maintenance of Cell Polarity in Yeast. In preparation

Santos, B. and Snyder, M. (2000). Sbe2p and Sbe22p, Two Homologous Golgi Proteins. Involved in Yeast Cell Wall Formation. Mol. Biol. Cell, in press.

Kumar, A., Cheung, K.H., Ross-Macdonald, P., Coelho, P.S.R., Miller, P. and Snyder, M. (2000) TRIPLES: A Database of Gene Function in S. cerevisiae. Submitted.

Kumar, A. des Etages, S.A. Coelho, P.S.R., Roeder, G.S. and Snyder, M. (1999) High-Throughput Methods for the Large-Scale Analysis of Gene Function by Transposon Tagging. Methods Enzymol. In press
des Etages, Kumar, A. and Snyder, M. (1999). Transposons as Tools. In press

Last updated 2001